منابع مشابه
DNaseR: DNase I footprinting analysis of DNase-seq data
The combination of DNase I digestion and high-throughput sequencing (DNaseseq) has been used recently to map chromatin accessibility in a given tissue or cell type on a genome-wide scale (Song and Crawford, 2010). In addition to DNase I hypersensitive sites (DHSs), short regions of protected nucleotides known as footprints can be detected using a technique known as ”digital genomic footprinting...
متن کاملMeasuring Arabidopsis chromatin accessibility using DNase I-polymerase chain reaction and DNase I-chip assays.
DNA accessibility is an important layer of regulation of DNA-dependent processes. Methods that measure DNA accessibility at local and genome-wide scales have facilitated a rapid increase in the knowledge of chromatin architecture in animal and yeast systems. In contrast, much less is known about chromatin organization in plants. We developed a robust DNase I-polymerase chain reaction (PCR) prot...
متن کاملDNase-I-dependent dissociation of erythrocyte cytoskeletons
The human erythrocyte contains a complex of peripheral membrane proteins which forms an extensive network or cytoskeleton on the cytoplasmic membrane surface. When I treat erythrocyte cytoskeletons with deoxyribonuclease I (DNase I), the cytoskeletons dissociate and erythrocyte actin is solubilized. The dissociation of the cytoskeletons by DNase I parallels the disruption of actin filaments in ...
متن کاملAnti-DNA antibodies bind to DNase I
Polyspecificity is a well-known property of the anti-DNA antibodies produced by autoimmune animals. In our search for antigen targets of anti-DNA antibodies within tissue extracts, we identified a 32-kD polypeptide that was recognized by a large panel of anti-DNA antibodies. Direct sequencing of this protein disclosed its identity with DNase I. 22 monoclonal anti-DNA antibodies bound to DNase I...
متن کاملDigestion of insect chromatin with micrococcal nuclease, DNase I and DNase I combined with single-strand specific nuclease S1.
The chromatin of the lepidopteran Ephestia kuehniella was digested by micrococcal nuclease, DNase I and S1-nuclease combined with DNase I pretreatment. The resulting DNA fragments were analyzed by gel electrophoresis and compared with the DNA fragments of rat liver nuclei obtained by the same process. Extensive homology was revealed between insect and mammalian chromatin structure. The combined...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 1988
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.28.87